A protein that breast, lung and other cancers use to promote their spread–or metastasis–to the brain, has been identified by a team led by Weill Cornell Medicine and New York-Presbyterian investigators.
The protein, CEMIP, will now be a focus of efforts to predict, prevent and treat brain metastases, which are a frequent cause of cancer deaths.
In their study, published in Nature Cell Biology, the scientists found that CEMIP prompts blood vessel and resident immune cells in the brain to produce inflammatory molecules, which in turn support the survival and progression of cancer cells to form brain tumours.
In lab-dish and animal-model experiments, removing CEMIP greatly impeded this brain metastasis process.
In tests on human patients’ breast and lung tumours, the researchers linked high CEMIP levels to a high risk of metastasis to the brain.
“Our findings suggest that blocking CEMIP could be a good strategy for preventing or treating brain metastasis, and that monitoring CEMIP levels in primary tumours might enable us, for the first time, to predict the risk of brain metastasis,” said co-senior author Dr. David Lyden, the Stavros S. Niarchos Professor in Pediatric Cardiology, and a professor of paediatrics and of cell and developmental biology at Weill Cornell Medicine.
The vast majority of cancer deaths are caused not by the initial, primary tumours that form but by secondary, metastatic tumours in vital organs.
Brain metastases are the most lethal of these and are also relatively common, occurring in an estimated 150,000 to 200,000 cancer patients each year in the United States. There has been no good way to predict these metastases, let alone prevent or treat them.
In the study, the scientists found that CEMIP, a protein previously linked to some cancers, is produced at high levels by brain-metastasising human breast and lung cancer cells.
CEMIP is concentrated at especially high levels in these cells’ “exosomes,” tiny capsules that are secreted by cells and can circulate in the body and be taken up by other cells. In contrast, CEMIP tends to be produced at relatively low levels by cancer cells that metastasise to bones and other organs.
The team found evidence that CEMIP-containing exosomes from primary breast and lung tumours are taken up mostly by endothelial cells that make up the blood vessels in the brain, and by nearby brain-resident immune cells called microglial cells.
The protein triggers changes in these cells including the increased production of inflammatory molecules that previously have been linked to metastasis.
“Even when we deleted the CEMIP gene from the cancer cells, providing CEMIP through exosomes restored the molecular environment in brain tissue that allowed the cancer cells to colonise that tissue,” said first author Gonçalo Rodrigues, a Ph.D. student co-mentored by Dr. Lyden and Dr. Maria de Sousa, emerita professor at the University of Porto in Portugal and adjunct professor of paediatrics at Weill Cornell Medicine. Dr. de Sousa is also a co-senior author of the study.
In the absence of CEMIP, the scientists found, cells from brain-metastasis-prone cells failed to colonise brain tissue normally in tissue-culture experiments and in animal models.
Co-senior author Dr. David Pisapia, an assistant professor of pathology and laboratory medicine at Weill Cornell Medicine and a pathologist at NewYork-Presbyterian/Weill Cornell Medical Center, examined over 300 samples from primary and metastatic tumours of breast and lung cancer patients, and found that primary tumours associated with brain metastases tended to have much higher levels of CEMIP compared to primary tumours associated with bone or other metastases, or no metastases. The researchers linked higher CEMIP production in the primary tumour to a faster progression to brain metastasis, while higher CEMIP production in brain metastases correlated with shorter survival times for the patients.
The results suggest that the level of CEMIP in biopsied tumour tissue and exosomes might be a useful biomarker of brain metastasis risk. “Paradoxically, due to improved therapies and prolonged survival times an ever increasing proportion of patients develop metastases in the brain, where tumour cells, protected by the blood-brain barrier, are ‘hiding’ from therapies. Therefore, it would be great to have a tool to identify patients that are most at risk for developing brain metastases post-standard treatments,” said Dr. Lyden, who is also a member of the Sandra and Edward Meyer Cancer Center and the Gale and Ira Drukier Institute for Children’s Health at Weill Cornell Medicine.
Source: Weill Corneal Medicine