Researchers discover possible way to make resistant melanomas susceptible to immunotherapy

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A Ludwig Cancer Research study has uncovered a cellular mechanism by which melanomas that fail to respond to checkpoint blockade may be made susceptible to immunotherapies. Led by Ping-Chih Ho of the Lausanne Branch of the Ludwig Institute for Cancer Research and reported in the current issue of Nature Immunology, the study also identifies an existing diabetes drug that could be used to accomplish this feat.

Checkpoint blockade therapies lift the brakes imposed by the body on the immune response, prompting an attack on tumours by the immune system’s killer T cells. Although these therapies have notched up successes against some major cancers—most notably melanoma and lung cancer—they fail to induce responses in many patients. For example, more than 40% of melanoma patients fail to benefit from even a combination of anti-PD1 and anti-CTLA4 checkpoint blockade therapies.

“We know that when patients don’t respond, the main reason is that their tumours are not infiltrated with T cells,” says Ho.

“It’s like the immune system doesn’t have soldiers on the battle field, so it can’t engage in the battle.”

Wan-Chen Cheng, a graduate student in Ho’s lab and the first author of the study, began by examining what differentiates gene expression in such “cold” tumours from that of their “hot”, T cell-infiltrated counterparts. A computational analysis of the genes expressed by melanomas profiled in The Cancer Genome Atlas (TCGA)—a public repository of genomic and clinical information on a wide variety of tumours—revealed that tumours that elicit robust anti-cancer immune responses also tend to express high levels of a metabolic protein named UCP2.

Gene expression patterns suggested UCP2-expressing melanoma tumours also express a subset of “cytokines” that draw immune cells into their microenvironment, particularly the killer T cell and the conventional type 1 dendritic cell (cDC1). The latter can prime and boost the killer T cell attack on sick cells. Further analysis of TCGA data suggested that tumours expressing UCP2 appear to be infiltrated with killer T cells and cDC1 cells.

To test these inferences, Cheng, Ho and their team grafted into mice melanoma tumours that could be prompted to express high levels of UCP2. Inducing UCP2 expression indeed boosted the production of factors that drive anti-cancer responses and drew a flood of killer T cells and cDC1 cells into the tumours. Tumours in mice engineered to lack cDC1 cells lacked such T cell infiltration, even when UCP2 was overexpressed.

“With these studies we confirmed that the expression of this protein by cancer cells can change the immune status of the tumour microenvironment, and that this precipitates a well-known anti-tumour immune cycle that is controlled by cDC1 and killer T cells,” says Ho.

The researchers next engrafted mice with melanoma tumours known to resist anti-PD-1 checkpoint blockade. Inducing UCP2 expression in these tumours and treating the mice with an anti-PD1 drug elicited robust anti-tumour immune responses that significantly extended survival of the mice. The enhanced immunity also seemed to be restricted to the tumour microenvironment, attenuating a risk associated with checkpoint-blockade: the provocation of autoimmune responses that destroy healthy tissues, sometimes to debilitating and even lethal effect.

Scouring the literature, the researchers also identified a diabetes drug named rosiglitazone that has been reported to induce UCP2 expression. Treating tumour-bearing mice with this drug similarly turned cold tumours hot, sensitized them to checkpoint blockade and extended survival. Notably, the team found the drug induced UCP2 expression in cultures of melanoma cells obtained from patients as well.

“Our results suggest that drugs that activate this pathway might improve the therapeutic outcomes of checkpoint blockade treatment,” says Ho.

The researchers are also confirming their results in preclinical studies that could support a trial evaluating the use of rosiglitazone to improve checkpoint blockade therapy for melanoma.


Paper: Wan-Chen Cheng et al, Uncoupling protein 2 reprograms the tumour microenvironment to support the anti-tumour immune cycle, Nature Immunology (2019). DOI: 10.1038/s41590-018-0290-0

Source: Ludwig Institute for Cancer Research

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